NCI-H460 is a tumorigenic cell line that was established in 1982 from the lung tissue pleural fluid of a male patient with large cell lung carcinoma. NCI-H460 cells exhibit an epithelial morphology and adherent cultural properties. The NCI-H460 cell line is also hypotriploid and has a modal chromosome number of 57. NCI-H460 cells express p53 mRNA at levels similar to those of normal lung tissue and stain positively for kertain and vimentin. NCI-H460 is a suitable transfection host and an NCI-H460 transfection kit can be purchased from Altogen Biosystems to conduct lung cancer research.